ABSTRACT: Generally, primary methods of identification as the fingerprint analysis, dental analysis or DNA examination are indicated for the establishment of the identity of a corpse. In situations with poor body conservation, such as in advanced stage of putrefaction or skeletonization, imaging exams like medical and dental computed tomography can assist in the process of identification. The frontal sinuses present anatomical characteristics that allow the establishment of the identity of an in dividual. In this case report we used the technique of three-dimensional construction of the frontal sinuses through the generation of solid figures representative of the sinus morphology. After the comparative analysis of the antemortem and postmortem tomography of the alleged victim, we could establish similarities in both the variations in size, shape, symmetry and contour of borders, and the presence and number of septa, allowing us to infer that the two images described belong to the same individual, thus establishing the identity of the corpse found.

RESUMEN: Generalmente, los métodos primarios de identificación como el análisis dactiloscópico, el análisis dental o el examen de ADN están indicados para el establecimiento de la identidad de un cadáver. En situaciones de mala conservación del cuerpo, como en etapa avanzada de putrefacción o esqueletización, los exámenes de imagen como la tomografía computarizada médica y dental pueden ayudar en el proceso de identificación. Los senos frontales presentan características anatómicas que permiten establecer la identidad de un individuo. En este reporte de caso utilizamos la técnica de construcción tridimensional de los senos frontales a través de la generación de figuras sólidas representativas de la morfología sinusal. Tras el análisis comparativo de la tomografía antemortem y postmortem de la presunta víctima, pudimos establecer similitudes tanto en las variaciones de tamaño, forma, simetría y contorno de márgenes, como en la presencia y número de septos, lo que nos permite inferir que las dos imágenes descritas pertenecen al mismo individuo, estableciéndose así la identidad del cadáver encontrado.

Abstract Background: To assess the prevalence and clinical relevance of anti-Jo-1 autoantibodies in a representative sample of patients with definite dermatomyositis (DM). Methods: This retrospective cohort study took place from 2005 to 2020 and assessed 118 adult patients from a tertiary center who were diagnosed with definite DM. A commercial kit was used to detect anti-Jo-1 autoantibodies. Results: The presence of anti-Jo-1 autoantibodies was observed in 10 out of 118 (8.5%) patients with definite DM. The following variables were comparable between individuals with and without anti-Jo-1 autoantibodies: age at diagnosis, sex, ethnicity, disease duration, follow-up period, recurrence rate, complete clinical response, death rate, and cancer incidence. There was no difference in clinical features between groups, except for an increased prevalence of “mechanic's hands,” joint involvement, and lung disease, as well as a reduced occurrence of skin findings in patients positive for anti-Jo-1 autoantibodies. No anti-Jo-1-positive patients went into remission; they required greater use of glucocorticoids and immunosuppressive drugs. Conclusions: Anti-Jo-1 positivity was found in 8.5% of patients with definite DM. This autoantibody was associated with an antisynthetase syndrome phenotype and might predict clinical outcomes in patients with definite DM.

Abstract Muscle overuse and its consequent muscle damage has no cure. Therefore, the present study aimed to investigate the regulatory role of tau-AuNPs on muscle recovery of muscle overuse model. The animals (Male Swiss mice) were randomly divided into four groups: Control (Ctr; n=6); tau-AuNPs (n=6); overuse (n=6); and overuse plus tau-AuNPs (n=6). Exercise sessions were performed for 21 consecutive days, and one exercise model was applied daily in the following sequence: low intensity, moderate intensity, and high intensity. The mice were then sacrificed. The quadriceps muscles were surgically removed for subsequent biochemical analysis (oxidative stress parameters, DNA damage markers and muscle differentiation protein). The overuse group significantly increased the oxidative stress parameters and DNA damage markers, whereas tau-AuNPs significantly decreased the oxidative stress parameters in the overuse animal model. However, there were no significant differences observed between overuse group and overuse plus tau-AuNPs administrated group in relation to DNA damage markers including DNA damage frequency and index levels when compared to control and tau-AuNPs groups. Muscle differentiation protein Myf-5 was increased in the overuse plus tau-AuNPs administration group when compared to control group. In conclusion, tau-AuNPs had significant effect on reducing oxidative stress parameters and increasing myogenic regulatory protein Myf-5 in the overuse group. However, it did not have significant effect on reducing DNA damage.

RESUMO: Em que medida a evasão e as experiências discentes nos cursos de graduação em Física estão relacionadas à pressão por excelência acadêmica? Baseados em leituras da sociologia da educação, realizamos uma análise de dados coletados no curso de Física de uma universidade brasileira. As evidências indicam que estudantes de famílias privilegiadas tendem a abandonar o curso, mas os que permanecem costumam ser bem-sucedidos. Ao mesmo tempo, a evasão não se relaciona com o desempenho acadêmico: há estudantes excelentes que desejam sair, bem como estudantes de baixo desempenho que desejam permanecer. A austeridade da organização curricular contribui para que os estudantes tenham lacunas em sua formação. Com algumas exceções, os professores são descritos como figuras opressoras, exigentes e sem empatia. Por outro lado, relações competitivas são percebidas entre os próprios estudantes. Enfim, ainda que a evasão não tenha relação com o desempenho acadêmico, a busca por excelência estrutura as experiências dos estudantes no curso.

ABSTRACT: To what extent are dropout and students’ experiences in undergraduate Physics courses related to pressure for academic excellence? Drawing on sociology of education, we performed an analysis of data collected from the Physics course at a Brazilian public university. Evidence indicates that students from privileged families tend to drop out, but those who remain tend to be successful. At the same time, dropout is not related to academic performance: there are excellent students who want to leave, as well as underperforming students who want to stay. Curricular austerity contributes to students having gaps in their education. With few exceptions, teachers are described as oppressive, demanding, and merciless figures. On the other hand, competitive relationships are perceived among the students themselves. Finally, even though dropout is not related to academic performance, the urge for excellence structures students’ experiences in the course.

RESUMEN. ¿En qué medida la deserción y las experiencias de los estudiantes de la carrera de Física están relacionadas con la presión por excelencia académica? Basados en lecturas de la sociología de la educación, realizamos un análisis de los datos recogidos en la carrera de Física de una universidad brasileña. Las evidencias apuntan que los estudiantes procedentes de familias privilegiadas se inclinan a dejar la carrera, pero los que permanecen suelen ser exitosos. A la vez, la deserción no se relaciona con el rendimiento académico: hay estudiantes excelentes que desean dejar la carrera, bien como hay estudiantes de bajo rendimiento que desean permanecer. La austeridad de la organización curricular contribuye a que los estudiantes tengan lagunas en su formación. Con algunas excepciones, los profesores son descriptos como figuras opresoras, exigentes y sin empatía. Por otro lado, se perciben relaciones competitivas entre los propios estudiantes. Por fin, aunque la deserción no tenga relación con el rendimiento académico, la búsqueda por excelencia diseña las experiencias de los estudiantes en carrera.

Resumo: A falta de integração acadêmica e social tem sido considerada importante para explicar as razões institucionais que produzem evasão. Neste artigo, investigamos as relações entre a integração dos estudantes do Instituto de Física da Universidade de Brasília e sua origem social. Um instrumento baseado no modelo de Tinto foi elaborado e aplicado a uma amostra representativa do corpo discente (n = 160). A evidência indica que: (1) entre os ingressantes, a ocorrência de motivação para a docência é independente da motivação para pesquisa; (2) os estudantes intelectualmente mais confiantes são os mesmos que reportam um convívio mais regular e agradável com os colegas; (3) os estudantes de periferia tendem a ser menos integrados social e academicamente, ainda que não existam diferenças motivacionais significativas entre eles e os estudantes mais privilegiados ao ingressar no curso. Em conjunto, os resultados recomendam um olhar mais sensível aos desdobramentos das diferenças sociais sobre as relações humanas nos Institutos de Física.

Abstract: The lack of academic and social integration has been considered important to explain the institutional reasons for student dropout. In this paper, we investigate the relationship between the integration of students of the Institute of Physics of UnB and their social background. An instrument based on the Tinto model was elaborated and applied to a representative sample of the student body (n = 160). Evidence indicates that: (1) among the freshmen, the occurrence of motivation for teaching is independent of the motivation for research; (2) we should suspect the belief that enjoying social life competes with academic success, since intellectually confident students are also those who report more regular and enjoyable social life with peers; (3) outskirt students tend to be less socially and academically integrated. Taken together, the results recommend a sensitive look at the unfolding of social differences on human relations in the Physics Institutes.

Abstract In this work, the divergence and curl operators are obtained using the coordinate free non rigid basis formulation of differential geometry. Although the authors have attempted to keep the presentation self-contained as much as possible, some previous exposure to the language of differential geometry may be helpful. In this sense the work is aimed to late undergraduate or beginners graduate students interested in mathematical physics. To illustrate the development, we graphically present the eleven coordinate systems in which the Laplace operator is separable. We detail the development of the basis and the connection for the cylindrical and paraboloidal coordinate systems. We also present in [1] codes both in Maxima and Maple for the spherical orthonormal basis, which serves as a working model for calculations in other situations of interest. Also in [1] the codes to obtain the coordinate surfaces are given.

RESUMO Objetivo: evidenciar a estrutura, funcionamento e contribuições das reuniões de trabalho da Estratégia Saúde da Família para o planejamento local em saúde. Método: estudo de caso único com cinco unidades integradas de análise, representadas pela coordenadora do Centro de Saúde e quatro equipes da Estratégia Saúde da Família. A coleta de dados ocorreu por meio de entrevistas semiestruturadas, observação não participante e pesquisa documental no período de outubro a dezembro de 2014. Os dados foram triangulados, organizados com o software MaxQDA®Plus, e analisados segundo a técnica de construção da explanação. Resultados: identificaram-se três categorias analíticas: Estrutura e funcionamento das reuniões de trabalho da Estratégia Saúde da Família; Participação dos profissionais nas reuniões de trabalho da Estratégia Saúde da Família para o planejamento local; Processos que contribuem para o planejamento local em saúde. Conclusão: as reuniões de trabalho são momentos indispensáveis para construção do planejamento local em saúde. Proporcionam a integração dos profissionais por meio de discussões, facilitando a tomada de decisões e a troca de informações.

ABSTRACT Objective: to demonstrate the structure, functioning and contributions of the Estratégia Saúde da Família work meetings regarding local health planning. Method: a single case study with five integrated units of analysis, represented by the coordinator of the Health Centre and four Estratégia Saúde da Família teams. Data collection was performed through semi-structured interviews, non-participant observation and documentary research from October to December 2014. Data were triangulated, organized with MaxQDA®Plus software, and analyzed according to the explanatory construction technique. Results: three analytical categories were identified: Structure and functioning of the Estratégia Saúde da Família work meetings; Participation of professionals in the Estratégia Saúde da Família work meetings for local planning; Processes that contribute to local health planning. Conclusion: work meetings are essential for the construction of local health planning. They provide professional integration through discussions, and facilitate decision-making and information exchange.

RESUMEN Objetivo: evidenciar la estructura, funcionamiento y contribuciones de las reuniones de trabajo de la Estratégia Saúde da Família para la planificación local en salud. Método: estudio de caso único con cinco unidades integradas de análisis, representadas por la coordinadora del Centro de Salud y cuatro equipos de la Estratégia Saúde da Família. La recolección de datos ocurrió a través de entrevistas semiestructuradas, observación no participante e investigación documental en el período de octubre a diciembre de 2014. Los datos fueron triangulados, organizados con software MaxQDA®Plus, y analizados según técnica de construcción de la explicación. Resultados: se identificaron tres categorías analíticas: Estructura y funcionamiento de las reuniones de trabajo de la Estratégia Saúde da Família; Participación de los profesionales en las reuniones de trabajo de la Estratégia Saúde da Família para la planificación local; Procesos que contribuyen a la planificación local en salud. Conclusión: las reuniones de trabajo son momentos indispensables para la construcción de la planificación local en salud. Proporcionan la integración de los profesionales a través de discusiones, facilitando la toma de decisiones y el intercambio de informaciones.

ABSTRACT Objective: to demonstrate the structure, functioning and contributions of the Estratégia Saúde da Família work meetings regarding local health planning. Method: a single case study with five integrated units of analysis, represented by the coordinator of the Health Centre and four Estratégia Saúde da Família teams. Data collection was performed through semi-structured interviews, non-participant observation and documentary research from October to December 2014. Data were triangulated, organized with MaxQDA®Plus software, and analyzed according to the explanatory construction technique. Results: three analytical categories were identified: Structure and functioning of the Estratégia Saúde da Família work meetings; Participation of professionals in the Estratégia Saúde da Família work meetings for local planning; Processes that contribute to local health planning. Conclusion: work meetings are essential for the construction of local health planning. They provide professional integration through discussions, and facilitate decision-making and information exchange.

RESUMEN Objetivo: evidenciar la estructura, funcionamiento y contribuciones de las reuniones de trabajo de la Estratégia Saúde da Família para la planificación local en salud. Método: estudio de caso único con cinco unidades integradas de análisis, representadas por la coordinadora del Centro de Salud y cuatro equipos de la Estratégia Saúde da Família. La recolección de datos ocurrió a través de entrevistas semiestructuradas, observación no participante e investigación documental en el período de octubre a diciembre de 2014. Los datos fueron triangulados, organizados con software MaxQDA®Plus, y analizados según técnica de construcción de la explicación. Resultados: se identificaron tres categorías analíticas: Estructura y funcionamiento de las reuniones de trabajo de la Estratégia Saúde da Família; Participación de los profesionales en las reuniones de trabajo de la Estratégia Saúde da Família para la planificación local; Procesos que contribuyen a la planificación local en salud. Conclusión: las reuniones de trabajo son momentos indispensables para la construcción de la planificación local en salud. Proporcionan la integración de los profesionales a través de discusiones, facilitando la toma de decisiones y el intercambio de informaciones.

RESUMO Objetivo: evidenciar a estrutura, funcionamento e contribuições das reuniões de trabalho da Estratégia Saúde da Família para o planejamento local em saúde. Método: estudo de caso único com cinco unidades integradas de análise, representadas pela coordenadora do Centro de Saúde e quatro equipes da Estratégia Saúde da Família. A coleta de dados ocorreu por meio de entrevistas semiestruturadas, observação não participante e pesquisa documental no período de outubro a dezembro de 2014. Os dados foram triangulados, organizados com o software MaxQDA®Plus, e analisados segundo a técnica de construção da explanação. Resultados: identificaram-se três categorias analíticas: Estrutura e funcionamento das reuniões de trabalho da Estratégia Saúde da Família; Participação dos profissionais nas reuniões de trabalho da Estratégia Saúde da Família para o planejamento local; Processos que contribuem para o planejamento local em saúde. Conclusão: as reuniões de trabalho são momentos indispensáveis para construção do planejamento local em saúde. Proporcionam a integração dos profissionais por meio de discussões, facilitando a tomada de decisões e a troca de informações.

Abstract INTRODUCTION: Chronic hepatitis C is a leading cause of liver disease. Infection triggers an immediate immune response in the host that is mediated by humoral/cellular mechanisms. T cells respond to infection via secretion of cytokines, which inhibit or stimulate one another, leading to cytokine imbalance and ultimately affecting treatment. Studies using interferon (IFN) and ribavirin (RBV) showed that TCD8+ cells and cytokine levels are associated with sustainable virological response (SVR). However, studies that investigated the effects of triple therapy (TT) are limited. METHODS: The study included hepatitis C virus (HCV)+ RNA, naives, genotype 1, ≥18 years, and advanced fibrosis (F≥3) patients. Samples were collected at baseline and after 12 weeks (W12) of TT. Six cytokines were analyzed by flow cytometry. RESULTS: Of 31 patients, four were excluded (two deaths, one interrupted TT, and one F2 patient). Of the 27 remaining patients, 21 (78%) were cirrhotic. SVR was achieved in 63% of the patients. The patients had a mean age of 55.11 ± 10.03 years. Analyses at baseline showed that the chemokine CCL5/Regulated on Activation, Normal T Cell Expressed and Secreted (RANTES) (p=0.04) and interleukin (IL)-6 (p=0.02), which was associated with SVR. RANTES (p=0.04) and IL-8 (p=0.01) levels were associated with SVR at W12. CONCLUSIONS Similar to patterns observed during double therapy, IL-6, IL-8, and RANTES levels were associated with SVR in TT, indicating the potential role of interferon in immune response to hepatitis C virus.

RESUMO CONTEXTO: A infecção pelo vírus da hepatite C (VHC) é uma das principais causas de morbidade e mortalidade relacionada ao fígado, através de sua evolução para cirrose hepática, complicações hepáticas em estágio terminal e carcinoma hepatocelular. Atualmente, os novos fármacos para a infecção pelo VHC, baseados nos novos antivirais de ação direta (AADs), modificaram os resultados nesse cenário. OBJETIVO: Avaliar a incidência de morte, durante a espera pelo tratamento com as novas drogas, e analisar quais variáveis independentes (idade, sexo, ascite, HDA, albumina, α-fetoproteína, plaquetas e escore de MELD) tiveram relação com o óbito. MÉTODOS: Estudo prospectivo com pacientes cirróticos pelo VHC. Inclusão: pacientes cirróticos por biópsia hepática (METAVIR), clínica ou imagem, RNA detectável (VHC). Exclusão: Outras fases de fibrose hepática e carcinoma hepatocelular. Estatística descritiva em variáveis contínuas. Exato de Fisher e Kaplan Meier e Análise de Regressão de Cox para avaliar a associação das variáveis estudadas com o óbito. P<0,05. RESULTADOS: Um total de 129 pacientes foram incluídos. Destes, 73% eram homens. A idade média foi de 57,8±12,1, a albumina de 3,5±0,6 mg/dL, as plaquetas de 123,4±59,6 e o escore de MELD de 10,59±3,56. O tempo de observação foi de 11,2±3,26 meses e o número de mortes 9/129 (6,9%). O Kaplan-Meier mostrou associação entre o óbito com albumina menor que 2,9 (0,0006), escore MELD maior que 15 (0,007) e α-fetoproteína maior que 40 ng/mL (<0,0001). A análise de regressão de Cox ajustada mostrou que α-fetoproteína maior que 40 ng/mL poderia ser considerada um risco independente para morte. CONCLUSÃO: Concluímos que pacientes com cirrose avançada devem ser priorizados para tratamento com AADs.

ABSTRACT BACKGROUND: The infection for the hepatitis C virus (HCV) is a leading cause of liver-related morbidity and mortality through its evolution to liver cirrhosis, end-stage liver complications and hepatocellular carcinoma. Currently, the new drugs for the HCV infection, based on direct antiviral agents, have changed the outcomes in this setting. OBJECTIVE: To assess death incidence, during the wait for the treatment with the new drugs, and to analyze which independent variable (age, sex, ascite, HDA, albumin, α-fetoprotein, platelets and Meld score) had relation with death. METHODS: Prospective study with cirrhotic patients by HCV. Inclusion: cirrhotic patients by hepatic biopsy (METAVIR), clinic or image, detectable RNA (HCV). Exclusion: Other stages of hepatic fibrosis and hepatocellular carcinoma. Descriptive statistic in continue variables. Fisher Exact and Kaplan Meier and Cox Regression Analysis to assess the association of variables studied with death. P<0.05. RESULTS: A total of 129 patients were included. Of this, 73% were men. Mean age was 57.8±12.1, albumin of 3.5±0.6 mg/dL, platelets of 123.4±59.6 and Meld score of 10.59±3.56. The time of observation was 11.2±3.26 months, and the number of death 9/129 (6,9%). The Kaplan-Meier showed association between death with albumin lower than 2.9 (0.0006), MELD score higher than 15 (0.007) and α-fetoprotein higher than 40 ng/mL (<0.0001). Adjusted Cox Regression Analysis showed that α-fetoprotein higher than 40 ng/ml could be considered an independent risk for death. CONCLUSION: We conclude that, patients with advanced cirrhosis should be prioritized for treatment with direct antiviral agents.

Abstract Hibiscus acetosella was shown to exert beneficial effects in humans and animal models however, the effects of this plant on DNA are unknown. The aim of this study was to determine the antigenotoxic and antimutagenic effects of H. acetosella extracts on alkylating agent methyl methanesulfonate (MMS) in vivo in mice. Initially, we performed analysis of phenolic compounds in extracts of H. acetosella by high-performance liquid chromatography (HPLC). Next, mice were divided into 8 groups and treated with distilled water or plant extract (0.1 ml/10 g) by gavage for 15 days, followed by intraperitoneal (ip) administration of saline solution or MMS (40 mg/Kg b.w) on day 16. Caffeic acid, following by gallic acid, gallocatechin, coumaric acid, and 3,4-dihydroxybenzoic acid were found to be present in extracts of H. acetosella leaves. In peripheral blood analysis of groups receiving pretreatment with H. acetosella at doses of 50 or 100 mg/kg plus MMS decreased DNA damage as evidenced by comet assay and Micronucleus assays relative to MMS alone. These results suggested that H. acetosella extracts exerted protective effects dose dependent against genotoxicity and mutagenicity induced by alkylating agents.

RESUMO CONTEXTO: A infecção pelo vírus da hepatite C (VHC) é um grave problema de saúde pública, que afeta aproximadamente 170 milhões de pessoas no mundo. A infecção crônica pelo VHC está associada à resistência à insulina hepática e a um risco aumentado de diabetes. Os doentes infetados pelo VHC foram bem documentados. OBJETIVO: Avaliar o modelo de avaliação da homeostase do índice de resistência à insulina (HOMA-IR) em pacientes tratados com medicação antiviral de ação direta na resposta virológica sustentada (RVS), categorizada pela presença ou ausência de cirrose. MÉTODOS: Foi realizado um estudo prospectivo. Os dados foram coletados no início do tratamento (t-base) e na décima segunda semana após o término do tratamento (t-RVS12). Os critérios de inclusão foram presença de: infecção pelo VHC (RNA-VHC positivo), idade ≥18 anos, conclusão da terapia de antivirais de ação direta e presença de diabetes com uso de hipoglicemiantes orais. Todas as amostras foram coletadas durante o período do estudo. Os critérios de exclusão foram: presença de coinfecção VHB/HIV, carcinoma hepatocelular no início do estudo, pacientes diabéticos em uso de insulina e pacientes transplantados (fígado/rim). A fibrose foi avaliada por elastografia hepática ou biópsia (METAVIR). A cirrose foi determinada por resultados clínicos ou exames de imagem. O HOMA-IR foi calculado como insulinemia de jejum (μU/mL) x glicemia de jejum (mmol/L) /22,5). Os pacientes foram divididos em dois grupos: a população geral do estudo (todos os pacientes, incluindo os diabéticos) e a população especial (pacientes com valores normais de HOMA-IR, que é <2,5 e sem diabetes). O valor do delta HOMA-IR foi calculado como: HOMA-IR no t-base - HOMA-IR no t-RVS12. Para a análise estatística descritiva, foram utilizados o teste t pareado e o modelo linear generalizado, assumindo a função de ligação logarítmica. Um valor de P<0,05 foi considerado significativo. RESULTADOS: Foram incluídos 150 pacientes e 75 eram cirróticos. A idade média foi de 55,3±9,97 e o índice de massa corpórea foi de 27,4±5,18. Vinte e dois (14,67%) eram pacientes diabéticos em uso de hipoglicemiantes orais e 17 (11%) eram cirróticos. Na população geral do estudo, os valores médios de glicose e HOMA-IR aumentaram na t-SVR12, mas a insulina diminuiu. O delta HOMA-IR foi negativo em t-SVR12, mas não houve diferença significativa. Excluindo pacientes diabéticos e aqueles com valores normais de HOMA-IR (<2,5), a média de glicose, insulina e HOMA-IR diminuiu no t-RVS12. O delta HOMA-IR diminuiu significativamente em t-RVS12 (P: 0,02). CONCLUSÃO: Na população geral, os valores de glicose e HOMA-IR aumentaram no t-RVS12, mas a insulina diminuiu. Na população especial, glicose, insulina, HOMA-IR e delta HOMA-IR diminuíram no t-RVS12.

ABSTRACT BACKGROUND: Hepatitis C virus (HCV) infection is a serious public health problem, that affects approximately 170 million people worldwide. Chronic HCV infection is associated with hepatic insulin resistance and an increased risk of diabetes HCV-infected patients has been well documented. OBJECTIVE: To assess the homeostasis model assessment of insulin resistance (HOMA-IR) index in patients treated with direct acting antiviral (DAAs) medication in the sustained virological response (SVR), categorized by the presence or absence of cirrhosis. METHODS: A prospective study was conducted. Data were collected at the beginning of treatment (t-base) and in the twelfth week after the completion of treatment (t-SVR12). The inclusion criteria were presence of: HCV infection (RNA-HCV positive), age ≥18 years, completion of DAAs’ therapy, and presence of diabetes with use of oral hypoglycemic agents. All samples were collected during the study period. The exclusion criteria were: presence of HBV/HIV co-infection, hepatocellular carcinoma at baseline, diabetic patients taking insulin and transplanted patients (liver/kidney). Fibrosis was assessed by hepatic elastography or biopsy (METAVIR). Cirrhosis was determined by clinical results or imaging. HOMA-IR was calculated as fasting insulin (μU/mL) × fasting glucose (mmol/L)/22.5) The patients were divided into two groups: the general study population (all patients, including the diabetic patients) and the special population (patients with normal values of HOMA-IR, which is >2.5, and without diabetes). The delta HOMA-IR value was calculated as: HOMA-IR at t-base - HOMA-IR at t-SVR12. For the descriptive statistical analysis, the paired t-test and generalized linear model assuming the log binding function were performed. A P value of < 0.05 was considered significant. RESULTS: We included 150 patients, and 75 were cirrhotic. The mean age was 55.3±9.97 and body mass index was 27.4±5.18. Twenty-two (14.67%) were diabetic patients using oral hypoglycemic agents, and 17 (11%) were cirrhotic. In the general study population, the mean glucose and HOMA-IR values increased at t-SVR12, but insulin decreased. Delta HOMA-IR was negative at t-SVR12, but there was no significant difference. Excluding diabetic patients and those with normal HOMA-IR values (<2.5), mean glucose, insulin and HOMA-IR decreased at t-SVR12. Delta HOMA-IR decreased significantly at t-SVR12 (P: 0.02). CONCLUSION: In the general population, glucose and HOMA-IR values increased at t-SVR12, but insulin decreased. In the special population, glucose, insulin, HOMA-IR and Delta HOMA-IR decreased at t-SVR12.

ABSTRACT Creatine acts intracellularly as energy buffer and storage, demonstrating protective effects in animal models of neurodegenerative diseases. However, its permeability throught blood-brain barrier (BBB) is reduced. The aim of the present study was developing a carrier to facilitate the delivery of creatine to the central nervous system. Creatine nanoliposomes were produced, characterized and assayed in models of toxicity in vitro and in vivo. Particles showed negative zeta potential (-12,5 mV), polydispersity index 0.237 and medium-size of 105 nm, which was confirmed by transmission electron microscopy (TEM) images. Toxicity assay in vitro was evaluated with blank liposomes (no drug) or creatine nanoliposomes at concentrations of 0.02 and 0.2 mg/mL, that did not influence the viability of Vero cells. The result. of the comet assay that the nanoliposomes are not genotoxic, togeher with cell viability demonstrated that the nanoliposomes are not toxic. Besides, in vivo assays not demonstrate toxicity in hematological and biochemical markers of young rats. Nevertheless, increase content of creatine in the cerebral cortex tissue after subchronic treatment was observed. Altogether, results indicate increase permeability of creatine to the BBB that could be used as assay for in vivo studies to confirm improved effect than free creatine.

A new fluorophore containing 2,1,3-benzothiadiazole unit with alkylated tetrazole terminal groups as electron donors connected through a π-conjugated system of the type (D-π-A-π-D) was synthesized using the Sonogashira coupling reaction with good yields. The genotoxicity of the two intermediate compounds and the final product was evaluated in vitro in consideration of the potential application of the new compound in the chemical production of photovoltaic paints. The new fluorophore did not show significant biological effects in the cell viability test and the comet assay when compared with the intermediate compounds that were obtained synthetically despite the ability to interact with deoxyribonucleic acid (DNA) as demonstrated in circular dichroism assays. Thus, the compound does not show toxic or genotoxic activity, and therefore, it can be applied as a pigment in photovoltaic paint.

RESUMO CONTEXTO: A interação entre lípides séricos e infecção pelo vírus C já é bem conhecida, assim como o comportamento dos níveis séricos daqueles durante o tratamento com Peg-IFN/RBV. No entanto, com antivirais de ação direta (AADs) este comportamento ainda não está claro. OBJETIVO: Comparar os níveis séricos de lípides entre pacientes tratados com Peg-IFN/RBV e AADs e avaliar os lípides na resposta virológica sustentada (RVS) com AADs. MÉTODOS: Estudo retro prospectivo comparando o comportamento dos níveis séricos de colesterol total (CT), lipoproteínas de baixa densidade (LDL) e triglicérides (TG) durante o tratamento com AADs (G-AADs) e um grupo histórico de controle Peg-IFN/RBV (G-PR). Coorte, estudo prospectivo, para estudar o comportamento dos lípides na RVS com AADs. Os dados foram coletados no início do tratamento (baseline: t-base) e na décima segunda semana de tratamento (t-12) para G-AADs e na vigésima quarta semana de tratamento (t-24) para G-PR para a análise comparativa entre os dois grupos. Na avaliação de coorte, as amostras foram coletadas no t-base e na décima segunda semana após o término do tratamento (t-RVS). Delta lípides: diferença entre lípides em t-12/t-24 menos t-base para comparação entre os grupos G-PR e G-AADs e t-RVS menos t-base para análise de lípides na RVS. A análise estatística descritiva, os testes não paramétricos de Kruskal Wallis e Wilcoxon foram utilizados para comparar o delta lípides dos grupos. O valor de P considerado foi de 0,05. RESULTADOS: Na avaliação entre os grupos G-PR e G-AADs, incluímos 63 e 121 pacientes, respectivamente. Os grupos não diferiram um do outro (IMC, sexo, genótipo, fibrose, colesterol total, LDL e TG), exceto por idade (50,38±10,44 vs 56±9,69, P=0,0006). Observamos uma diminuição nos níveis de CT e LDL e um aumento de TG no G-PR, no G-AADs ocorreu o oposto (Δ CT -13,9±34,5 vs 4,12±34,3 P=0,0005, Δ LDL -7,16±32 vs 10,13±29,92, P=0,003, Δ TG 4,51±53,7 vs -8,24±49,93, P=0,0025). Na análise de coorte, foram incluídos 102 pacientes, 70% homens e 56% F4. Noventa e cinco deles atingiram a RVS. Observamos um aumento de CT e LDL e uma diminuição de TG em ambos os grupos (RVS e não RVS), sem diferença estatística (Δ CT P=0,68; Δ LDL P=0,69; Δ TG P=0,43). Não encontramos diferença significativa na avaliação dos deltas pelos genótipos 1 e 3 (Δ CT +29,7±40,2 vs +13,4±30,3, P=0,06; Δ LDL + 21,4±28,6 vs +16,6±31,3, P=0,41; Δ TG -3,6±60,6 vs -0,7±40, P=0,91). CONCLUSÃO: O nível de lípides séricos diferiu durante o tratamento com Peg-IFN/RBV e AADs. O tratamento com AADs foi associado a um aumento de CT e LDL e uma diminuição de TG, independentemente da RVS.

ABSTRACT BACKGROUND: The interaction between serum lipids and C virus infection is well known, as are serum lipid levels in the Peg-IFN / RBV-based treatment. However, with direct action antivirals (DAAs) this behavior is still unclear. OBJECTIVE: To compare serum lipids levels between patients treated with Peg-IFN/RBV and DAAs and to evaluate lipids in sustained virological response (SVR) with DAAs. METHODS: Retro prospective study comparing the behavior of total cholesterol (TC), low-density lipoprotein (LDL) and triglycerides (TG) serum levels during treatment with DAAs (G-DAAs) and a control historic group Peg-IFN/RBV (G-PR). Coorte, prospective study, to study the behavior of lipids in the SVR with DAAs. Data were collected at the beginning of treatment (baseline: t-base) and at week 12 of treatment (t-12) for G-DAAs and at week 24 (t-24) for G-PR, groups. In the cohort evaluation, the samples at t-base and at week 12 after the end of treatment (t-SVR). Delta lipids: difference between lipids in t-12 / t-24 minus t-base for comparison between G-PR and G-AADs groups and t-SVR minus t-base for lipid analysis in SVR. Analysis with Kruskal Wallis and Wilcoxon tests to compare the delta lipids of the groups. The P value was 0.05. RESULTS: In the assessment between G-PR and G-DAAs groups, we included 63 and 121 patients, respectively. The groups did not differ one from the other (BMI, sex, genotype, fibrosis, total cholesterol, LDL, and TG) except by age (50.38±10.44 vs 56±9.69, P=0.0006). We observed a decrease in levels of TC and LDL and an increase in TG, in G-PR, and in G-DAAs the opposite (Δ TC -13.9±34.5 vs 4.12±34.3 P=0.0005, Δ LDL -7.16±32 vs 10.13±29.92, P=0.003, Δ TG 4.51±53.7 vs -8.24±49.93, P=0.0025). In the coorte analysis, we included 102 patients, 70% men and 56% F4, 95 of them reached SVR. We observed an increase of TC and LDL and a decrease of TG in both groups (SVR and non SVR), with no statistical difference (Δ TC P=0.68; Δ LDL P=0.69; Δ TG P=0.43). We did not find significant difference in delta evaluation by genotype 1 and 3 (Δ TC +29.7±40.2 vs +13.4±30.3, P=0.06; Δ LDL +21.4±28.6 vs +16.6±31.3, P=0.41; Δ TG -3.6±60.6 vs -0.7±40, P=0.91). CONCLUSION: Serum lipids level differed during treatment with Peg-IFN and DAAs. Treatment with DAAs was associated with an increase of TC and LDL and a decrease of TG, independently of SVR.